Biotinylated Protein Ladder Detection Pack 7727S from Cell Signaling Technology

Product Specs
ItemBiotinylated Protein Ladder Detection Pack
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number7727S
Quantity650 µl
Conjugate/Tag/LabelBiotin
Research AreaRelated Products
Molecule NameBiotinylated Protein Ladder Detection Pack
Nucleic Acid TypeProtein Ladder
Features (standardized)Unstained, Tagged
Size Range9 - 200 kDa
TypeProtein Ladder

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) NADPH oxidase 1: A target in the capacity of dimeric ECG and EGCG procyanidins to inhibit colorectal cancer cell invasionRedox BiologySeptember 1, 2023Wei Zhu, Patricia I. OteizaHRP-conjugated (#7074), anti-rabbit biotinylated (#14708), streptavidin (#3999) and the biotinylated ladder (#7727) were from Cell Signaling Technology, Inc. (Danvers, MA). The primary antibody for NOX1 (#ab78016) was

(2) Targeting Phospholipase D Pharmacologically Prevents Phagocytic Function Loss of Retinal Pigment Epithelium Cells Exposed to High Glucose LevelsInternational Journal of Molecular SciencesOctober 5, 2022Vicente BermÃºdez, Paula EstefanÃa Tenconi, MarÃa Sol EchevarrÃa, Aram Asatrian, Jorgelina Muriel Calandria, Norma MarÃa Giusto, Nicolas Guillermo Bazan, Melina Valeria MateosPrecision Plus Protein Dual Color Standards (#1610374. Bio-Rad Life Science group) and Biotinylated Protein Ladder Detection Pack (#7727, Cell Signaling Technology, Inc) in the case of ABC cell Western Blots. 4.8. Real-Time Quantitative PCR (qPCR

(3) Biallelic loss-of-function variants in WDR11 are associated with microcephaly and intellectual disabilityEuropean journal of human genetics : EJHGNovember 1, 2021Haag N, Tan EC, Begemann M, Buschmann L, Kraft F, Holschbach P, Lai AHM, Brett M, Mochida GH, DiTroia S, et al.were used. PageRuler Prestained (ThermoScientific) and biotinylated Protein Ladders (Cell Signaling, 7727S) were used for protein molecular weight estimation. Detection was done using Clarity Western ECL Substrate

(4) A Role for TGFβ Signaling in Preclinical Osteolytic Estrogen Receptor-Positive Breast Cancer Bone Metastases ProgressionInternational Journal of Molecular SciencesApril 24, 2021Julia N. Cheng, Jennifer B. Frye, Susan A. Whitman, Andrew G. Kunihiro, Ritu Pandey, Janet L. FunkDanvers, MA, USA), or Biotinylated Protein Ladder followed by anti-biotin HRP-linked secondary antibody (#7727, CST, Danvers, MA, USA), were used to estimate probed-proteins ≤190 kDa in molecular weight, and Precision

(5) Evaluation of [Cys(ATTO 488)8]Dermorphin-NH2 as a novel tool for the study of μ-opioid peptide receptorsPLOS ONEApril 23, 2021Despina Giakomidi, Mark F. Bird, John McDonald, Erika Marzola, Remo Guerrini, Serena Chanoch, Nidhuna Sabu, Barbara Horley, Girolamo Calo, David G. Lambertheated to 95˚C for 5min. 50μl of each sample, along with protein marker (biotinylated protein ladder #7727 Cell signalling technology) was loaded onto an 10% SDS-PAGE gel. Proteins were separated by electrophoresis

(6) Hexameric procyanidins inhibit colorectal cancer cell growth through both redox and non-redox regulation of the epidermal growth factor signaling pathwayRedox BiologyJanuary 1, 2021Elena Daveri, Ana M. Adamo, Eugenia Alfine, Wei Zhu, Patricia I. Oteizasecondary antibodies anti-rabbit HRP-conjugated (#7074) or biotinylated (#14708) and biotinylated ladder (#7727) were from Cell Signaling Technology, Inc. (Danvers, MA, USA). Primary antibodies against α-tubulin

(7) SON DNA‐binding protein mediates macrophage autophagy and responses to intracellular infectionFEBS LettersSeptember 1, 2020David J. Gregory, Glen M. DeLoid, Sharon L. Salmon, Dennis W. Metzger, Igor Kramnik, Lester Kobzikgel (Invitrogen) with Biotinylated Molecular Weight Marker (Cell Signalling Technologies catalogue 7727). See Table 2 for antibodies. Images were acquired on an HD2 gel imager (Alpha Innotech) with ALTA U

(8) Multiple novel hepatocellular carcinoma signature genes are commonly controlled by the master pluripotency factor OCT4Cell Oncol (Dordr)April 1, 2020Chao Ye et al.secondary antibody (7074), normal Rabbit IgG (2729) and a biotinylated protein ladder detection pack (7727) were all purchased from Cell Signaling Technology. An anti-GAPDH (AG019) antibody was purchased from

(9) Combating viral contaminants in CHO cells by engineering innate immunityScientific ReportsDecember 1, 2019Austin W. T. Chiang et al.(Thermo Fisher Scientific) under reducing conditions. As a molecular weight marker, protein ladder (cat# 7727S) from Cell Signaling Technology (Danvers, MA) was used. Nitrocellulose membranes and iBlot™ transfer

(10) Transcriptomic changes triggered by ouabain in rat cerebellum granule cells: Role of α3- and α1-Na+,K+-ATPase-mediated signalingPLOS ONESeptember 26, 2019Larisa V. Smolyaninova, Alexandra A. Shiyan, Leonid V. Kapilevich, Alexander V. Lopachev, Tatiana N. Fedorova, Tatiana S. Klementieva, Aleksey A. Moskovtsev, Aslan A. Kubatiev, Sergei N. Orlovmembrane. Biotinylated proteins served as molecular weight markers (Cell Signaling Technologies, Cat#7727). The membranes were blocked and incubated overnight with primary and appropriate secondary HRP-conjugated

(11) Transcription Factor IRF4 Dysfunction Affects the Immunosuppressive Function of Treg Cells in Patients with Primary Immune ThrombocytopeniaBioMed research internationalJanuary 1, 2019Tang M, Cheng L, Li F, Wu B, Chen P, Zhan Y, Hua F, Min Z, Ke Y, Liu C, et al.and combined with biotin labeled protein molecular weight standard (Cell Signaling Technology, Cat# 7727) 10 𝜇l per lane. After electrophoresis of protein power transferred to nitrocellulose membrane (Cell Signaling Technology

(12) Exceptionally high-affinity Ras binders that remodel its effector domainJournal of Biological ChemistryMarch 1, 2018John H. McGee, So Youn Shim, Seung-Joo Lee, Paige K. Swanson, Sam Y. Jiang, Michael A. Durney, Gregory L. VerdineHealth) using a biotinylated ladder and anti-biotin-HRP (Cell Signaling Technology, catalog numbers 7727 and 7075). Nuclear magnetic resonance To prepare 15N-labeled KRas or miniprotein, cells were grown

(13) Loss of inversin decreases transepithelial sodium transport in murine renal cellsAmerican journal of physiology. Cell physiologyDecember 1, 2017Kulkarni NH, Smith RC, Blazer-Yost BL.analysis [anti-rabbit IgG conjugated to horseradish peroxidase (HRP) no. 7074S] and biotinylated protein ladder (no. 7727) were from Cell Signaling Technology (Beverly, MA). Cell culture. The mouse cortical collecting duct

(14) Proteomic analysis of affinity-purified extracellular proteasomes reveals exclusively 20S complexesOncotargetNovember 24, 2017Valentina A. Kulichkova, Tatiana O. Artamonova, Olga G. Lyublinskaya, Mikhail A. Khodorkovskii, Alexey N. Tomilin, Anna S. TsimokhaBio-Rad Laboratories) and analyzed by Western blotting, using antibodies against biotin (HRP-conjugate, #7727, 1:2500, Cell Signaling Technology) and primary antibodies to α-tubulin mouse (T6074, 1:5000), β-tubulin

(15) Histone Hypervariants H2A.Z.1 and H2A.Z.2 Play Independent and Context-Specific Roles in Neuronal Activity-Induced Transcription of Arc/Arg3.1 and Other Immediate Early GeneseNeuroJuly 1, 2017Dunn CJ, Sarkar P, Bailey ER, Farris S, Zhao M, Ward JM, Dudek SM, Saha RN.

(16) Novel GM-CSF signals via IFN-γR/IRF-1 and AKT/mTOR license monocytes for suppressor functionBlood advancesJune 13, 2017Ribechini E, Hutchinson JA, Hergovits S, Heuer M, Lucas J, Schleicher U, Jordán Garrote AL, Potter SJ, Riquelme P, Brackmann H, et al.peroxidase (HRP) (#7074), P-4E-BP1 (#2855P), p-mTOR (#5536P), anti-mouse-HRP (#7076), and anti-biotin-HRP (#7727) (all Cell Signaling), Rel A p65 (clone F-6, Santa Cruz), NF-kB p100 (polyclonal rabbit, Cell Signaling),

(17) Activation of transient receptor potential vanilloid 4 involves in hypoxia/reoxygenation injury in cardiomyocytesCell death & diseaseMay 25, 2017Wu QF, Qian C, Zhao N, Dong Q, Li J, Wang BB, Chen L, Yu L, Han B, Du YM, et al.electrophoresis gels, and 10 μl biotinylated protein ladder (Cell Signaling Technology, Danvers, MA, USA, #7727) loaded into a separate lane. Then, it was transferred to a nitrocellulose membrane by Electrophoresis

(18) Cleavage of the urokinase receptor (uPAR) on oral cancer cells: regulation by transforming growth factor - β1 (TGF-β1) and potential effects on migration and invasionBMC cancerMay 19, 2017Magnussen SN, Hadler-Olsen E, Costea DE, Berg E, Jacobsen CC, Mortensen B, Salo T, Martinez-Zubiaurre I, Winberg JO, Uhlin-Hansen L, et al.The PNGase F kit (P0704S) was from New England BioLabs (Beverly, MA). Biotinylated protein ladder (7727, lot: 21) was from Cell Signaling Technology (Danvers, MA). Western blotting Luminol Reagent (sc2048)

(19) Progestin increases the expression of gonadotropins in pituitaries of male zebrafishJournal of EndocrinologyJuly 1, 2016Cuili Wang, Dongteng Liu, Weiting Chen, Wei Ge, Wanshu Hong, Yong Zhu, Shi X Chendetermined by comparing blotted protein size to a biotinylated protein ladder (Cell Signaling Technology, #7727s) following the manufacturer’s directions. Expression of fshb and lhb mRNA during the diurnal cycle To

(20) Administration of Gemcitabine After Pancreatic Tumor Resection in Mice Induces an Antitumor Immune Response Mediated by Natural Killer CellsGastroenterologyMay 12, 2016Engin Gürlevik, Bettina Fleischmann-Mundt, Jennifer Brooks, Ihsan Ekin Demir, Katja Steiger, Silvia Ribback, Tetyana Yevsa, Norman Woller, Arnold Kloos, Dmitrij Ostroumov, Nina Armbrecht, Michael P. Manns, Frank Dombrowski, Michael Saborowski, Moritz Kleine, Thomas C. Wirth, Helmut Oettle, Güralp O. Ceyhan, Irene Esposito, Diego F. Calvisi, Stefan Kubicka, Florian Kühnel

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Biotinylated Protein Ladder Detection Pack

Biotinylated Protein Ladder Detection Pack from Cell Signaling Technology

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Cell Signaling Technology

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Biotinylated Protein Ladder Detection Pack from Cell Signaling Technology

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